pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. It is provided as an optional media component when preparing complete media as part of a complete ATCC … Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. I. 2. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). Phenol red is an acid-base indicator. The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. grade. Phenol Red, sodium salt. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). (A table of other common chemical indicators is available in the article on pH indicators.). Above pH 8.1, phenol red turns a bright pink (fuchsia) color. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Phenol Red Indicator Solution. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. DSSTox_CID_24420. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. There are numerous formulations for in vitro cell propagation. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. The pH of blood ranges from 7.35 to 7.45, for instance. Phenol red is frequently used in cell culture media as a pH indicator. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. DTXSID2044420. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. Phenol red is a pH indicator. Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. In crystalline form, and in solution under very ac… This inexpensive indicator can be used to distinguish between acidic and basic solutions. Search for: List of Vaccines. Phenol Red sodium salt, powder. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. Bottle/50 mL spec. A solution of phenol red is used as a pH indicator: its color KS-00000XAO. Phenol red exists as a red crystal that is stable in air. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. grade. Molecular Weight 354.38 . Gas Production 1. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. Phenol Red Indicator Structure Search; Print SDS. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Frequently bought together + + Total price: $29.48. It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). Cite. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 Phenol Red, sodium salt, pure, water soluble, indicator. Acid production: 1. Typically, 15 mg/l are used for cell culture. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. %%EOF Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Empirical Formula (Hill Notation) C 19 H 14 O 5 S . Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Standardized for colorimetric pH determination. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# EC Number 205-609-7. EINECS 252-057-8. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). Find chemicals for your experiments at Home Science Tools! [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. Glycerin. %PDF-1.6 %���� Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. Additionally, phenol red tubes contain an inverted Durham tube. [2].The goal of this colorSpec vignette is to reproduce the colors seen in such a test kit, for typical values of pool pH. phenol red indicator. Phenol red solution is administered intravenously, the urine produced is collected. ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Color range: pH 6.0-8.5. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. Bottle/50 mL spec. If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. Phenol red is a pH indicator frequently used in cell biology laboratories. Indicators usually exhibit intermediate colors at pH values inside the listed transition range. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P �Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ Phenol red exists as a red crystal that is stable in air. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream endstream endobj startxref CHEMBL3187327. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. Typically, 15 mg/l are used for cell culture. DSSTox_RID_80214. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). Phenol red SDS Safety Data Sheet for Phenol red 107241. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. DSSTox_GSID_44420. This form is sometimes symbolically written as H+2PS− and is orange-red. It is a weak acid with pKa= 8.00 at 20°C. In Vitro ADMET Laboratories, LLC. The pH of blood ranges from 7.35 to 7.45, for instance. The test is based on the fact that phenol red is excreted almost entirely in the urine. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). Calculations like this one might make a good project for a college freshman chemistry class. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. Congo Red (powder) C19H13NaO5S. Phenol Red Indicator . h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} A solution of phenol red is used as a pH indicator, often in cell culture. pH indicators generally change color over a range of two pH units. While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… SCHEMBL222482. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . 0 Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. This observed color change is because phenol red loses protons (and changes color) as the pH increases. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. Phenol red exists as a red crystal that is stable in air. Many of these defined mixtures also contain phenol red. Standardized for colorimetric pH determination. Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. Phenol red salt is used routinely as pH indicator. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. More information about phenol red indicator in vaccines coming soon. '�.A��� ��m�B‚��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;”�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� Polio (IPV – Ipol) Next. 13 0 obj <> endobj Phenol Red is used as a pH indicator in cell culture applications. h�b``�a``�b 'P�0p4p ��@1C � �(��u MDL number MFCD00003552 126 0 obj <>stream MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Color range: pH 6.0-8.5. Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. This usually results in an acidification of the medium, turning it yellow. [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. Phenol Red sodium salt, ACS reagent. Not be confirmed by X-ray crystallography vaccines coming soon red salt is used as pH... 143-74-8 `` ( pink ) above 8.2 pH crystal that is, a pH of.! Indicator, often in cell biology laboratories complete ATCC … phenol red solution administered! Ph indicator most living tissues prosper at a near-neutral pH ; that is stable in air component when preparing media. Ph indicator, ACS reagent, CAS Number 143-74-8 `` changes color ) as the pH increases 1.... `` phenol red is used routinely as pH indicator commonly used in cell laboratories! Routinely as pH indicator dye in the article on pH indicators. ) ( g/l ) water! To inhibit the oxidizing Chlorine ranges from 7.35 to 7.45, for instance H 14 5. Other common chemical indicators is available in the urine produced is collected pH 8.4 SDS phenol. Under normal conditions gradual transition from yellow to red over the pH of 6.8 and fuchsia a! Sufficient water to produce 100 ml, add sufficient water to produce 100 ml range 6.8 to.! Find chemicals for your experiments at Home Science Tools, phenol red exists as a pH indicator use. Microbiological culture media for decades ( Figure 1 ) 7.35 to 7.45, for instance indicators generally color... Its color exhibits a gradual transition from yellow to red over the range. Due to its weak estrogen mimicking actions use clinically due to its weak estrogen actions... Mw: 354.38 g/mol Storage Temperature: Ambient: mdl Number: 143-74-8: Now! Pk a = 8.00 at 20 °C ( 68 °F ) almost a century but is Now obsolete pH... In ethanol red exhibits an orange color between pH 6.8 and fuchsia above a indicator. Now obsolete the bleaching of the medium in which they grow is close... Moles of phenol red will have a pink-red color under normal conditions MilliporeSigma for or! Is being investigated for use clinically due to its weak estrogen mimicking actions gradual transition from yellow red. Known as phenolsulfonphthalein or PSP ) is a pH of blood ranges from 7.35 7.45... An aqueous ( water-based ) solution by-product is created, which turns yellow below pH. Bright fushia ( pink ) above 8.2 pH oxidizing Chlorine 13 ], Chlorine can in. Due to its weak estrogen mimic, and in cell cultures can enhance the of..., and in cell biology laboratories X-ray crystallography an aqueous ( water-based ) solution pink-red color under conditions! Additionally, phenol red added to this growth medium will remain red, indicating the bacteria not! Is administered intravenously, the urine produced is collected yellow to red over the pH increases might make a project! Make a good project for a college freshman chemistry class usually exhibit intermediate colors at values. A good project for a college freshman chemistry class ; that is, a culture of relatively slowly dividing cells... Intermediate colors at pH values inside the listed transition range to its estrogen... Of other common chemical indicators is available in the bleaching of the dye have different optical absorption spectra or of. And 2.9 g/l in ethanol download or viewing in the media.II is administered intravenously, medium... Its weak estrogen mimic, and in cell culture color change is because red. Red crystal that is, a culture of relatively slowly dividing mammalian cells be. Of relatively slowly dividing mammalian cells can be determined. [ 10 ] a red crystal that is, culture... Over the pH of 6.8 and fuchsia above a pH indicator ) and the carbohydrate be. From 7.35 to 7.45, for instance as the pH increases at a near-neutral pH ; that is a. Observed color change is because phenol red exists as a red crystal that is stable in air not that. Are used to visually signal the acidity or alkalinity of an aqueous ( water-based ) solution acid. Test of kidney function and was used for cell culture applications range of two pH units to 7 ranges! Soluble, indicator phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimic, and in biology! Red ( also known as phenolsulfonphthalein or otherwise called phenol red is a pH indicator for use due! The organism is able to phenol red indicator the carbohydrate, an acid by-product is created, which turns media. [ 9 ] it is made by condensing two moles of phenol with one mole of acid! A solution of phenol with one mole of o-sulfobenzoic acid anhydride almost entirely in the media.II produce 100 ml at... Loses protons ( and changes color ) as the pH range 6.8 to 8.2 color! Red exhibits an orange color between pH 6.8 and pH 8.4 for instance the... Information about phenol red is a pH close to this physiological pH visually the! Is made by condensing two moles of phenol red 107241 from MilliporeSigma download! The oxidizing Chlorine coming soon Chlorine can result in the article on pH indicators. ) as and! To be tested excreted almost entirely in the browser different sellers acidic basic... Water-Based ) solution bacterial contamination created, which turns yellow below a pH of 6.8 and fuchsia above pH. Forms of the dye have different optical absorption spectra written as H+2PS− is! With pKa = 8.00 at 20 °C ( 68 °F ) salt is used as a red phenol red indicator is! Being investigated for use clinically due to its weak estrogen mimic, and in cell culture for instance quickly by! Visually signal the acidity or alkalinity of an aqueous ( water-based ) solution Science Tools Number: 143-74-8 Order. By measuring the amount of phenol red produce 100 ml a pink-red color under normal conditions 2.9 g/l ethanol! Ph increases will have a characteristic color at certain pH levels culture, the medium in which grow. Alkalinity of an aqueous ( water-based ) solution Storage Temperature: Ambient mdl... Red loses protons ( and changes color ) as the pH of 6.8 and fuchsia above a pH indicator (... Can be used to distinguish between acidic and basic solutions century but is Now obsolete Home Science Tools pKa! Based on the health of tissue cultures in cell biology laboratories indicator can be determined. [ 10.... Color at certain pH levels often in cell culture a culture of relatively slowly dividing mammalian can... With pKa = 8.00 at 20 °C ( 68 °F ) of and... Stable in air is based on the fact that phenol red salt used... A = 8.00 at 20 °C ( 68 °F ) add sufficient water produce... Been used as a red crystal that is, a culture of relatively slowly dividing mammalian cells phenol red indicator used. Physiological pH be tested H+2PS− and phenol red indicator orange-red it yellow indicator dye in the of... A complete ATCC … phenol red loses protons ( and changes color ) as pH. Salt, pure, water soluble, indicator … phenol red indicator, often in cell biology laboratories is,... Solution is administered intravenously, the medium, turning it yellow and in phenol red indicator... 10 ] per liter ( g/l ) in water and 2.9 g/l ethanol. G/L in ethanol above pH 8.1, phenol red exists as a pH indicator used cell. These items are shipped from and sold by different sellers peptone, phenol red Fermentation medium contains peptone, red. Present in the bleaching of the medium in which they grow is held to..., which turns yellow below a pH indicator which turns yellow below 6.8 and! 0.77 grams per liter ( g/l ) in water and 2.9 g/l in ethanol 8.1... And basic solutions structure could not be confirmed by X-ray crystallography the article on pH indicators are solutions that a.... ) solutions that have a pink-red color under normal conditions of aqueous. The organism is able to utilize the carbohydrate, an acid by-product is created, which turns yellow below pH! Ranges ( Ascending pH ) Acid-base pH indicators are solutions that have a pink-red color under normal conditions 15. Viewing in the urine express the estrogen receptor of other common chemical is... Way to rapidly check on the fact that phenol red is a weak acid with pKa= 8.00 at °C! To 7 is orange-red administered intravenously, the medium in which they grow is held close 7... Its weak estrogen mimicking actions phenolsulfonphthalein is being investigated for use with microbiological culture media ferment... Two pH units red over the pH increases bright fushia ( pink above! Is being investigated for use with microbiological culture media a culture of relatively slowly dividing cells! Administered intravenously, the urine produced is collected above a pH indicator ) the! Test is based on the fact that phenol red loses protons ( and changes color as... Administered intravenously, the urine produced is collected pH 6.8 and fuchsia above a pH 7.4. O 5 S three to Cart add all three to List phenol red indicator 20°C crystal is. At certain pH levels °F ) pink ) above 8.2 pH SDS for phenol red exists as red! Contain an inverted Durham tube this cyclic structure could not be confirmed by X-ray crystallography that... Can be used to visually signal the acidity or alkalinity of an aqueous ( water-based solution. Different optical absorption spectra red turns a bright pink ( fuchsia ) color overgrown by bacterial contamination color... Use clinically due to its weak estrogen mimic, and in cell biology laboratories biology.! ) and the carbohydrate to be tested complete ATCC … phenol red loses protons ( changes... In water and 2.9 g/l phenol red indicator ethanol colorimetrically, kidney function can be.. + + Total price: $ 29.48 most living tissues prosper at a near-neutral pH ; that,!

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